ICSI Center

Molecular Genetics Research Laboratory for Male Infertility / Advanced Micromanipulation Research Laboratory on Mammalian Gametes


Our field of interest is to device and execute research projects in reproductive biology. The main focus is on designing and carrying out research projects on the physiology of human gametes, spermatozoa, and oocytes in order to understand their maturation and interaction. Other fields of interest are the genetic basis responsible for spermatogenesis and to explore techniques for assessment of aneuploidy of eggs and sperm. Systematically device and organize follow-up studies to investigate the wellbeing of children born from assisted reproductive technologies (ART), IVF, ICSI, and those resulting from cryopreservation. Investigate methods to enhance the efficiency of cryopreservation procedures of human eggs.



Perspectives on the assessment of human sperm chromatin integrity.

          Gianpiero D. Palermo, Queenie V. Neri, Tyler Cozzubbo, Zev Rosenwaks

Intracytoplasmic Sperm Injection (ICSI) in Extreme Cases of Male Infertility.

          Gianpiero D. Palermo, Queenie V. Neri, Peter N. Schlegel, Zev Rosenwaks

Safety of intracytoplasmic sperm injection.

           Palermo GD, Neri QV, Rosenwaks Z.

Treatment of male infertility.

          Palermo GD, Kocent J, Monahan D, Neri QV, Rosenwaks Z.

Understanding the Spermatozoan.

          Neri QV, Hu J, Rosenwaks Z, Palermo GD.

Age does not adversely affect sperm retrieval in men undergoing microdissection testicular sperm extraction.

          Ramasamy R, Trivedi NN, Reifsnyder JE, Palermo GD, Rosenwaks Z, Schlegel PN.

Understanding fertilization through intracytoplasmic sperm injection (ICSI).

          Neri QV, Lee B, Rosenwaks Z, Machaca K, Palermo GD.

Severe testicular atrophy does not affect the success of microdissection testicular sperm extraction.

          Bryson CF, Ramasamy R, Sheehan M, Palermo GD, Rosenwaks Z, Schlegel PN.


Role of tissue digestion and extensive sperm search after microdissection testicular sperm extraction.

Ramasamy R, Reifsnyder JE, Bryson C, Zaninovic N, Liotta D, Cook CA, Hariprashad J, Weiss D, Neri Q, Palermo GD, Schlegel PN.

Successful treatment of postchemotherapy azoospermia with microsurgical testicular sperm extraction: the Weill Cornell experience.

Hsiao W, Stahl PJ, Osterberg EC, Nejat E, Palermo GD, Rosenwaks Z, Schlegel PN.


What to do when ICSI fails.

Maggiulli R, Neri QV, Monahan D, Hu J, Takeuchi T, Rosenwaks Z, Palermo GD.


Treatment options for impaired spermatogenesis: germ cell transplantation and stem-cell based therapy.

Neri QV, Takeuchi T, Rosenwaks Z, Palermo GD.

Successful fertility treatment for Klinefelter's syndrome.

Ramasamy R, Ricci JA, Palermo GD, Gosden LV, Rosenwaks Z, Schlegel PN.

ICSI: where we have been and where we are going.

Palermo GD, Neri QV, Takeuchi T, Rosenwaks Z.

High serum FSH levels in men with nonobstructive azoospermia does not affect success of microdissection testicular sperm extraction.

Ramasamy R, Lin K, Gosden LV, Rosenwaks Z, Palermo GD, Schlegel PN.


Genetic and epigenetic characteristics of ICSI children.

Palermo GD, Neri QV, Takeuchi T, Squires J, Moy F, Rosenwaks Z.

Intracytoplasmic sperm injection (ICSI) using cryopreserved sperm from men with malignant neoplasm yields high pregnancy rates.

Hourvitz A, Goldschlag DE, Davis OK, Gosden LV, Palermo GD, Rosenwaks Z.

The outcome of intracytoplasmic sperm injection using occasional spermatozoa in the ejaculate of men with spermatogenic failure.

Bendikson KA, Neri QV, Takeuchi T, Toschi M, Schlegel PN, Rosenwaks Z, Palermo GD.

Male gamete empowerment.

Takeuchi T, Neri QV, Palermo GD.

An update of assisted reproductive technologies results in the United States.

Neri QV, Takeuchi T, Palermo GD.


Journal of Experimental & Clinical Assisted Reproduction: shaping the future of research and practice in reproductive endocrinology/infertility.

Sills ES, Winston RM, Palermo GD.

Intracytoplasmic sperm injection. Accomplishments and qualms.

Neri QV, Tanaka N, Wang A, Katagiri Y, Takeuchi T, Rosenwaks Z, Palermo GD.

Medical follow-up study of 5-year-old ICSI children.

Bonduelle M, Bergh C, Niklasson A, Palermo GD, Wennerholm UB; Collaborative Study Group of Brussels, Gothenburg and New York.

Implications of cloning technique for reproductive medicine.

Takeuchi T, Palermo GD.

Genetic and epigenetic modifications associated with human ooplasm donation and mitochondrial heteroplasmy - considerations for interpreting studies of heritability and reproductive outcome.

Sills ES, Takeuchi T, Tucker MJ, Palermo GD.

Y chromosome assessment and its implications for the development of ICSI children.

Katagiri Y, Neri QV, Takeuchi T, Schlegel PN, Megid WA, Kent-First M, Rosenwaks Z, Palermo GD.

A successful model to assess embryo development after transplantation of prophase nuclei.

Takeuchi T, Rosenwaks Z, Palermo GD.

AZF microdeletions of the Y chromosome and in vitro fertilization outcome.

Choi JM, Chung P, Veeck L, Mielnik A, Palermo GD, Schlegel PN.

Does ICSI require acrosomal disruption? An ultrastructural study.

Takeuchi T, Colombero LT, Neri QV, Rosenwaks Z, Palermo GD.


Assisted reproductive technologies and monozygous twins: implications for future study and clinical practice.

Sills ES, Tucker MJ, Palermo GD.

ICSI and its outcome.

Palermo GD, Neri QV, Hariprashad JJ, Davis OK, Veeck LL, Rosenwaks Z.

Use of electively cryopreserved microsurgically aspirated epididymal sperm with IVF and intracytoplasmic sperm injection for obstructive azoospermia.

Janzen N, Goldstein M, Schlegel PN, Palermo GD, Rosenwaks Z, Hariprashad J.

Correction of hyperinsulinemia in oligoovulatory women with clomiphene-resistant polycystic ovary syndrome: a review of therapeutic rationale and reproductive outcomes.

Sills ES, Perloe M, Palermo GD.

Xenogeneic transplantation of human spermatogonia.

Reis MM, Tsai MC, Schlegel PN, Feliciano M, Raffaelli R, Rosenwaks Z, Palermo GD.

Alternative sources of gametes: reality or science fiction?

Tsai MC, Takeuchi T, Bedford JM, Reis MM, Rosenwaks Z, Palermo GD.

Human zona pellucida micromanipulation and monozygotic twinning frequency after IVF.

Sills ES, Moomjy M, Zaninovic N, Veeck LL, McGee M, Palermo GD, Rosenwaks Z.


A comparison of human spermatozoa immunolabeling features using xenogenic reagents for centrosomal proteins.

Colombero LT, Takeuchi T, Sills ES, Breed WG, Rosenwaks Z, Palermo GD.

Sperm integrity is critical for normal mitotic division and early embryonic development.

Moomjy M, Colombero LT, Veeck LL, Rosenwaks Z, Palermo GD.

Incidence of sperm aneuploidy in relation to semen characteristics and assisted reproductive outcome.

Colombero LT, Hariprashad JJ, Tsai MC, Rosenwaks Z, Palermo GD.

The role of structural integrity of the fertilising spermatozoon in early human embryogenesis.

Colombero LT, Moomjy M, Sills ES, Rosenwaks Z, Palermo GD.

Isolation of the Ca2+ releasing component(s) of mammalian sperm extracts: the search continues.

Fissore RA, Reis MM, Palermo GD.

A reliable technique of nuclear transplantation for immature mammalian oocytes.

Takeuchi T, Ergün B, Huang TH, Rosenwaks Z, Palermo GD.

Fertilization and pregnancy outcome with intracytoplasmic sperm injection for azoospermic men.

Palermo GD, Schlegel PN, Hariprashad JJ, Ergün B, Mielnik A, Zaninovic N, Veeck LL, Rosenwaks Z.

Human glucosamine-6-phosphate isomerase, a homologue of hamster oscillin, does not appear to be involved in Ca2+ release in mammalian oocytes.

Wolny YM, Fissore RA, Wu H, Reis MM, Colombero LT, Ergün B, Rosenwaks Z, Palermo GD.

Testicular sperm extraction with intracytoplasmic sperm injection for nonobstructive azoospermia: testicular histology can predict success of sperm retrieval.

Su LM, Palermo GD, Goldstein M, Veeck LL, Rosenwaks Z, Schlegel PN.


Human sperm cytosolic factor triggers Ca2+ oscillations and overcomes activation failure of mammalian oocytes.

Palermo GD, Avrech OM, Colombero LT, Wu H, Wolny YM, Fissore RA, Rosenwaks Z.

Testicular sperm extraction with intracytoplasmic sperm injection for nonobstructive azoospermia.

Schlegel PN, Palermo GD, Goldstein M, Menendez S, Zaninovic N, Veeck LL, Rosenwaks Z.

The human sperm centrosome is responsible for normal syngamy and early embryonic development.

Palermo GD, Colombero LT, Rosenwaks Z.